CRISPRi-seq for genome-wide fitness quantification in bacteria

Vincent de Bakker, Xue Liu, Afonso M. Bravo, Jan-Willem Veening

Published: 2022-01-06 DOI: 10.1038/s41596-021-00639-6

CRISPRi-seq

genome-wide fitness

gene quantification

Streptococcus pneumoniae

single-guide RNA

AI 解读

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CRISPR interference (CRISPRi) is a powerful tool to link essential and nonessential genes to specific phenotypes and to explore their functions. Here we describe a protocol for CRISPRi screenings to assess genome-wide gene fitness in a single sequencing s
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
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References
ACKNOWLEDGMENTS

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CRISPRi-seq for genome-wide fitness quantification in bacteria

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