NGM Agar
Adrien Assie, Buck Samuel
Published: 2021-10-01 DOI: 10.17504/protocols.io.zy7f7zn
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Disclaimer
This is a standard protocol for C. elegans maintenance available on wormbook.
Abstract
NGM agar recipe from wormbook
Steps
1.
Start with 975mL
2.
3.0g NaCl
3.
2.5gPeptone
4.
17gAgar
5.
Autoclave with stir bar
6.
Cool to 55°C (faster in water bath)
7.
Then add the following while stirring on heat plate (DO NOT OVERHEAT)
8.
0.5mL of 1Molarity (M) CaCl2 (sterile)
9.
1mLof 5 mg/mL Cholesterol (dissolved in ethanol)
10.
1mL of 1Molarity (M) MgSO4 (sterile)
11.
25mL of 1Molarity (M) Potassium Phosphate Buffer, pH 6.0 (sterile)
12.
Pour into petri dishes using sterile technique
- 60 mm dishes = About
7mLper plate - 100 mm dishes = About
20mLper plate
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