This protocol collection is shared under the HTAN Internal Data and Materials Sharing Agreement and is provided as is. See section 14 of full agreement for full details.

This protocol is shared as an Open Access protocol (under HTAN's definitions). This protocol is Subject to IP Restrictions.

This protocol collection accompanies accompanies Expansion Sequencing (ExSeq), covering the four key steps of a targeted Expansion Sequencing (targeted ExSeq) experiment: (1) Padlock probe design; (2) tissue preparation and expansion; (3) library preparation; and (4) in situ sequencing with the Illumina chemistry.

For further details, consult the relevant protocols within the collection. These protocols were used to profile human metastatic breast cancer biopsies as a part of the Human Tumor Atlas Pilot Project (HTAPP).