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    YT medium

    Andreas Sagen

    Published: 2023-03-20 DOI: 10.17504/protocols.io.kxygx9qx4g8j/v1
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    Abstract

    2xYT is a nutritionally rich liquid microbial growth medium used for propagation of recombinant strains of Escherichia coli and M13 bacteriophage or other filamentous single-stranded DNA bacteriophages.

    Steps

    500 mL 2x YT medium

    1.

    Fill the bottle with 400mL deionized water

    2.

    Measure and add:

    `8g` Tryptone



`5g` Yeast extract



`2.5g` Sodium chloride

    Powdered compounds:

    3.

    Adjust pH to 7.0 by adding drops of concentrated NaOH

    4.

    Add deionized water to a total of 500mL

    5.

    Autoclave liquid at 121°C for 0h 15m 0s

    Note
    Cool to 50°C and supplement with antibiotics as appropriate

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